As enrolment took place over a wide interval from 14 to 32 weeks of gestation, we assessed the relation between gestational age at enrolment and development of pre eclampsia in women who received L arginine and antioxidant vitamins compared with placebo. Treatment before 24 weeks reduced the incidence of pre eclampsia (relative risk 0.37, 0.23 to 0.58), whereas treatment after 24 weeks did not (0.64, 0.30 to 1.37). However, when we estimated the interaction term between treatment effect and time of first treatment, we did not find it to be significant (B=0.002; P=0.96).

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